electroporation instrument (Bio-Rad)
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Bio-Rad
electroporation instrument
Electroporation Instrument, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 97/100, based on 5903 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/gene+pulser+instrument/Gene+Pulser+Xcell+Total+System/pmc12914205-114-0-3
Average 97 stars, based on 5903 article reviews
Electroporation Instrument, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 97/100, based on 5903 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/gene+pulser+instrument/Gene+Pulser+Xcell+Total+System/pmc12914205-114-0-3
Average 97 stars, based on 5903 article reviews
electroporation instrument - by Bioz Stars,
2026-09
97/100 stars
Images
1) Product Images from "Protocol to identify the signaling network of nucleotide second messengers in Shigella sonnei"
Article Title: Protocol to identify the signaling network of nucleotide second messengers in Shigella sonnei
Journal: STAR Protocols
doi: 10.1016/j.xpro.2026.104353
Figure Legend Snippet: Overview of the pKD46-Red-mediated scarless deletion of the target gene in S. sonnei ① The pKD46 plasmid (encoding Red recombinase) is pre-transformed into S. sonnei and cells are grown at 30 °C to competence. ② A PCR product containing antibiotic-resistance marker flanked by two FRT sites is amplified from pKD3/pKD4. ③ The targeting fragment is introduced by electroporation; Red-driven homologous recombination and antibiotic selection yield primary recombinants. ④ Cultivation at 42 °C cures the temperature-sensitive pKD46. ⑤ Introduction of the CP20 plasmid (encoding FLP recombinase) excises the resistance cassette between the FRT sites; a second 42 °C heat step eliminates CP20, leaving a clean, marker-free deletion mutant. H1/H2, homology extensions; P1/P2, primer sites; FRT, FLP recognition target. The figure is reprinted with permission from Wang et al., 2025. Created with BioRender.
Techniques Used: Plasmid Preparation, Transformation Assay, Marker, Amplification, Electroporation, Homologous Recombination, Selection, Mutagenesis
Figure Legend Snippet: Identification of the interaction between CRP and YdeH using the BACTH system Both crp and ydeH were cloned and inserted into the pUT18C and pKNT25 vectors, respectively, and co-transformed into the BTH101 strain by electroporation. The positive clones were spotted together with the negative control and positive control on the colour plate. Finally, the colour development of the plate was checked. Figure reprinted with permission from Wang et al., 2025. Created with BioRender.
Techniques Used: Clone Assay, Transformation Assay, Electroporation, Negative Control, Positive Control
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Transfection:Article Title: Expression and localization of grass carp pkc-θ (protein kinase C theta) gene after its activation. Article Snippet: Haemorrhagic disease caused by grass carp reovirus (GCRV) can result in large-scale death of young grass carp, leading to irreparable economic losses that seriously affect large-scale breeding.. Protein kinase C (PKC, also known as PRKC) represents a family of serine/threonine protein kinases that includes multiple isozymes in many species.. Among these, PKC-θ (PKC theta, also written as PRKCQ) is a novel isoform, mainly expressed in T cells, that is known to be involved in immune system function in mammals. Plasmid Preparation:Article Title: Expression and localization of grass carp pkc-θ (protein kinase C theta) gene after its activation. Article Snippet: Haemorrhagic disease caused by grass carp reovirus (GCRV) can result in large-scale death of young grass carp, leading to irreparable economic losses that seriously affect large-scale breeding.. Protein kinase C (PKC, also known as PRKC) represents a family of serine/threonine protein kinases that includes multiple isozymes in many species.. Among these, PKC-θ (PKC theta, also written as PRKCQ) is a novel isoform, mainly expressed in T cells, that is known to be involved in immune system function in mammals. Article Title: Structural and functional analysis of Bacillus cereus spore cortex lytic enzymes and YlaJ/YhcN lipoproteins Article Snippet: Verified pMAD plasmids were demethylated by passaging through dam-/dcm- E. coli (New England Biolabs, UK) before introducing to B. cereus by electroporation and selecting for transformants on LB plates supplemented with 1 μg ml −1 erythromycin, 5 μg ml −1 lincomycin and 90 μg ml −1 X-gal. .. Efficient electroporation was achieved using a Electroporation:Article Title: Expression and localization of grass carp pkc-θ (protein kinase C theta) gene after its activation. Article Snippet: Haemorrhagic disease caused by grass carp reovirus (GCRV) can result in large-scale death of young grass carp, leading to irreparable economic losses that seriously affect large-scale breeding.. Protein kinase C (PKC, also known as PRKC) represents a family of serine/threonine protein kinases that includes multiple isozymes in many species.. Among these, PKC-θ (PKC theta, also written as PRKCQ) is a novel isoform, mainly expressed in T cells, that is known to be involved in immune system function in mammals. Article Title: Unmasking Pathogen Traits for Chronic Colonization in Neurogenic Bladder Patients Article Snippet: .. Electroporation was conducted using the Article Title: Structural and functional analysis of Bacillus cereus spore cortex lytic enzymes and YlaJ/YhcN lipoproteins Article Snippet: Verified pMAD plasmids were demethylated by passaging through dam-/dcm- E. coli (New England Biolabs, UK) before introducing to B. cereus by electroporation and selecting for transformants on LB plates supplemented with 1 μg ml −1 erythromycin, 5 μg ml −1 lincomycin and 90 μg ml −1 X-gal. .. Efficient electroporation was achieved using a Article Title: Systematic evasion of the restriction-modification barrier in bacteria Article Snippet: Oligonucleotides were purchased from IDT Technologies 15 (Coralville, IA). .. Electroporation cuvettes (1 mm-gap) were purchased from BioRad and transformations 16 performed on a Article Title: Production of recombinant human G protein-coupled estrogen receptor (GPER) and establishment of a ligand binding assay using graphene quantum dots (GQDs) Article Snippet: .. Electroporation was performed using a Article Title: Statistical mining of triple-negative breast cancer-specific nanobodies among huge libraries from immunized alpacas Article Snippet: .. Electroporation-competent Escherichia coli TG1 cells (Agilent Technologies Japan, Ltd., Tokyo, Japan) were transformed with the ligated plasmids using the Transformation Assay:Article Title: Statistical mining of triple-negative breast cancer-specific nanobodies among huge libraries from immunized alpacas Article Snippet: .. Electroporation-competent Escherichia coli TG1 cells (Agilent Technologies Japan, Ltd., Tokyo, Japan) were transformed with the ligated plasmids using the Expressing:Article Title: Active Expression of Human Tissue Plasminogen Activator (t-PA) c-DNA from Pulmonary Metastases in the Methylotrophic Yeast Pichia Pastoris KM71H Strain Article Snippet: .. Preparation of the competent cells from the overnight culture was performed according to the work instruction of Pichia Expression Kit (Easy Select Pichia Expression Kit, Invitrogen), hence the competent cells were electroporated with 5μg of linearized pPICZalphaA-t-PA with PmeI (Thermo Scientific, Rochester, USA) at 37 0C for 16 h) using a |
